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HPLC Calibration

Dionex HPLC

Dionex is a leading brand specializing in high-performance liquid chromatography (HPLC) systems. These systems are highly regarded for their sensitivity, precision, and efficiency in separating, analyzing, and quantifying compounds in various industries such as pharmaceuticals, environmental testing, and chemical research

Key Features of Dionex HPLC Systems

High Sensitivity: Ideal for detecting trace amounts of substances in complex samples.
Automated Workflow: Integration with advanced software enables automation for sample preparation, analysis, and reporting.
Flexibility: Modular systems for customization based on analytical requirements.
Ion Chromatography Expertise: Specialization in ion chromatography for the analysis of ions and polar molecules.
Data Management: Equipped with Chromeleon software for data acquisition, regulatory compliance, and reporting.

HPLC Calibration

Calibration of HPLC systems is essential to ensure accurate, reliable, and consistent results. Proper calibration reduces errors and helps maintain the integrity of analytical methods. It typically involves calibrating key components like the pump, detector, and injector, as well as performing system suitability testing (SST).

Pump Calibration

Flow Rate Calibration: Verifies the flow rate of the mobile phase to match set values for accurate results.
Pressure Calibration: Ensures correct pressure readings to avoid deviations in chromatographic separation.

Detector Calibration

Wavelength Accuracy: Ensures the UV detector operates at specified wavelengths for proper detection.
Linear Response Calibration: Verifies the detector's linear response to concentration levels of analytes.

Injector Calibration

Injection Volume Accuracy: Ensures precise delivery of sample volumes into the column for quantification.
Carryover Check: Verifies that no contamination occurs between sample injections.

System Suitability Testing (SST)

System suitability testing evaluates the overall performance of the HPLC system by checking parameters such as resolution, peak symmetry, and retention time reproducibility. This ensures that the system is functioning correctly before starting analysis.

Calibration of HPLC systems, including Dionex machines, is vital for maintaining analytical accuracy and reliability. Routine calibration and maintenance help prevent errors, increase system longevity, and ensure compliance with regulatory standards.

Emergency Repairs and Calibration Services

We offer expert repair and calibration services for HPLC systems. Whether you need emergency repairs or routine calibration, our experienced technicians can provide fast, reliable support.

Conclusion

Proper calibration of your Dionex HPLC system is crucial for ensuring accuracy, reliability, and reproducibility of your analytical results. By regularly calibrating key components like the pump, detector, and injector, and performing system suitability testing, you can maintain the highest standards in your laboratory work. Whether you require routine maintenance, system repairs, or calibration services, ensuring your HPLC machine operates optimally is essential to the success of your analyses.